The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LCAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LCAT. Next,...

0.5mg/ml if reconstituted with 0.2ml sterile DI water. LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Lecithin Cholesterol Acyltransferase (LCAT) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of LCAT in mouse serum, plasma and other biological fluids. Detection Range: 1.56-100ng/ml, Sensitivity: 0.64ng/ml, Storage and Stability: Desiccation recommended. The...

Sample Type:, Serum, Plasma, Biological Fluids, Intended Use: This BioAssay(TM) kit is a sandwich ELISA for in vitro quantitative measurement of LCAT in Mouse serum, plasma and other biological fluids, Sensitivity: 0.375ng/mL, Range: 0.625-40ng/mL, Specificity: Mouse, Test Principle: This BioAssay(TM) ELISA kit uses...